Bioelectromagnetics
Reza Masoomi Jahandizi; Parviz Abdolmaleki; Seyed Javad Mowla
Volume 5, Issue 2 , June 2011, , Pages 105-115
Abstract
The effect of 15 and 30 mT of static magnetic field on the cell cycle of neural inductive rat BMSC was evaluated. The BMSC was inductived by neural inductive medium (NIM). Duration of inductive and Exposure time were 2, 4 and 6 hours. The cells induction to neural inductive medium associated with SMF ...
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The effect of 15 and 30 mT of static magnetic field on the cell cycle of neural inductive rat BMSC was evaluated. The BMSC was inductived by neural inductive medium (NIM). Duration of inductive and Exposure time were 2, 4 and 6 hours. The cells induction to neural inductive medium associated with SMF as exposed group, besides we have a control group. The apparatus we used to generate the SMF was a locally designed SMF generator in which there was an incubator instrument capable to maintain the humidity, temperature and CO2 concentration in predefined level. After exposing, the cells were fixed, stained and their percent of cell cycle phases; G1, S, G2/M were elucidated using flowcytometer instrument. The WinMdi 2.9 Software was used to process data from flowcytometer and elucidation of phase's percents. The results showed SMF with 15 mT intensity did not significantly alter the cell cycle in three different (2, 4 and 6 hours) exposing times. Exposing 2 hours with 30 mT increased the G2/M phases in neural inductive BMSC comparing to the corresponding control. Static magnetic field decreased the percent of S phase in BMSC, during 4 hours exposure.
Tissue Engineering
Jafar Ai; Saeed Sarkar; Mohammad Ali Oghabian
Volume 4, Issue 2 , June 2010, , Pages 161-166
Abstract
Various reviews have shown that strong electromagnetic fields have negative effects on human health. This study focused on the effect of MRI radiation on liver functional test histometery of liver in adult male rats. For this purpose, we used an MRI device that could produce 1.5 T electromagnetic radiations, ...
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Various reviews have shown that strong electromagnetic fields have negative effects on human health. This study focused on the effect of MRI radiation on liver functional test histometery of liver in adult male rats. For this purpose, we used an MRI device that could produce 1.5 T electromagnetic radiations, and chose 22 Wistar rats as laboratory animal models. Rats were divided into two equal groups. The first group exposed to 1.5T electromagnetic radiation and RF radiation during a 30- minute MRI scan as experimental group. The control group experienced 1.5T electromagnetic radiation exposure without RF radiation by the same MRI device. The rats were anesthetized and blood samples were obtained from cardiac chambers to measure the serum levels of LDL, HDL, ALT, AST, ALP, total cholesterol, total protein, albumin, total billirobin, and direct bilirobin. Livers were then removed and the specimens fixed. Serial sections (5 μm thick) were prepared from livers and the diameter of hepatocytes and their nuclei were measured. The findings of the present study indicate that, there was a significant increase (P<0.5) in amount of HDL, ALT, AST, ALP, total billirobin, direct bilirobin and there was a significant decrease (P<0.5) in amount of total cholesterol, LDL, total protein, and albumin in experimental group by comparison with control group. But no significant differences were seen in the diameter of hepatocytes and their nuclei between both groups. The electromagnetic radiations of MRI device may influence the level of liver enzymes and liver function without any histomorphologically changes. Conducting clinical trial studies with human subjects is recommended.
