نوع مقاله: مقاله کامل پژوهشی

نویسندگان

1 دانشجوی دکتری بیوفیزیک، گروه بیوفیزیک، دانشکده علوم زیستی، دانشگاه تربیت مدرس

2 دانشیار، گروه بیوفیزیک، دانشکده علوم زیستی، دانشگاه تربیت مدرس

3 دانشیار، گروه هماتولوژی، دانشکده پزشکی، دانشگاه تربیت مدرس

4 دانش‌آموختة کارشناسی ارشد، شرکت فناوری بن یاخته‌های رویان (بانک خون و بند ناف رویان)

5 دانش‌آموختة کارشناسی ارشد،گروه سلول‌های بنیادی و توسعة زیستی، مرکز تحقیقات علوم سلولی، پژوهشگاه رویان

10.22041/ijbme.2012.13103

چکیده

سلول‌های بنیادی مزانشیمی انسان که از بندناف نوزادان جدا و کشت داده شده بودند، به مدت 24 ساعت در معرض میدان مغناطیسی ایستا با شدت 24 میلی‌تسلا قرار گرفتند و درصد سلول‌های زنده و میزان پیشرفت در چرخة سلولی در نمونه‌های تابش دیده با شاهد مقایسه شد. نتایج نشان داد مجاورت با این میدان به مدت 24 ساعت در زمان‌های 36، 48 و60 ساعت پس از اعمال میدان سبب کاهش معنا‌داری در درصد سلول‌های زنده می‌شود. میزان پیشرفت این سلول‌ها در چرخه سلولی نیز، این یافته را تأیید کرد؛ اما بعد از گذشت 72 ساعت از زمان تابش‌دهی، این تغییر تا حد معناداری جبران شد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigating the impacts of Static Magnetic Field on cell viability and Cell Cycle Progression in Human Mesenchymal Stem cells

نویسندگان [English]

  • Maryam Sadri 1
  • Parviz Abdolmaleki 2
  • Saeed Abroun 3
  • Bahare Beiki 4
  • Fazel Samani 5

1 Ph.D Student, Biophysics Department, Faculty of Biological Sciences, Tarbiat Modares University

2 Associate professor, Biophysics Department, Faculty of Biological Sciences, Tarbiat Modares University

3 Associate professor, Hematology Department, Faculty of Medical Sciences, Tarbiat Modares University

4 M.Sc, Royan Stem Cell Technology Company (Royan Cord Blood Bank, R&D section

5 M.Sc, Department of stem cells and developmental biology, cell sciences research center, Royan Institute for Stem Cell Biology and Technology, ACECR

چکیده [English]

The Mesenchymal Stem cells derived from human newborn cords were cultured and exposed to a 24mT Static magnetic field for 24 hours. The viability percentage and the cell cycle progression was then investigated in exposed samples and the obtained results was compared with the control samples. The results clearly demonstrated a significant reduction of cell viability due to the exposure of 24 hours of SMF and post-exposure cultures within the time frames of 36,48,60 hours. The cell development through the cell-cycle, also verified this finding, however, 72 hours of post-exposure culture, significantly leveled off the drop in viable stem cell rates.

کلیدواژه‌ها [English]

  • Static magnetic fields
  • Mesenchymal stem cells
  • Cell cycle

[1] Ahlbom L., Cardis E., Green A., Review of the epidemiologic Literature on EMF and health; Environ. Health Perspect, 2001; 109:911-933.

[2] Ishisaka R., Kanno T., Inai Y., Nakahara H.,  Akiyama J., Yoshioka T., Utsumi K.,  Effect of a magnetic field on the various functions of subcellular organelles and cells;  Pathophysiology, 2000; 7: 149–152.

[3] Sergio Manzetti A., Johansson O., Global electromagnetic toxicity and frequency-induced diseases: Theoryand short overview; Pathophysiology, 2012; 19: 185–191.

[4]   Tavasoli Z. , Abdolmaleki P.,  Investigation the Effects of static Magnetic Field on Apoptosis in a Myeloid Cell Line K562; Proceeding of The 5th International Workshop on Biological Effects of Electromagnetic. Palermo, Italy, 2008.

[5]   Sarvestani A., Abdolmaleki P., Static Magnetic Fields Inhibit Radiation-Induced Apoptosis in Bone Marrow Stem Cells; Proceeding of The 5th International Workshop on Biological Effects of Electromagnetic. Palermo, Italy, 2008.

[6]   Qiu L. H., Tang X. N., Zhong M., Wang Z.Y.,  Effect of static magnetic field on proliferation and cell cycle of osteoblast cells; Shanghai Kou Qiang Yi Xue, 2004; 5: 469-70.

[7]    Walleczek J.,  Electromagnetic field effects on cells of the immune system  the role of calcium signaling; FASEB J. , 1992; 6: 3177-3185.

[8]   Hao Q., Wenfang C., Xia A., Qiang W., Ying L., et al., Effects of a Moderate-Intensity Static Magnetic Field and Adriamycin on K562 Cells;  Bioelectromagnetics, 2011; 32:191-199.

[9] Anne M., Hocking N., Gibran N.S., Mesenchymal stem cells: Paracrine signaling and differentiationduring cutaneous wound repair; Experimenal Cell Research  Arch, 2010; 316: 2213-2219.

[10] Mitalipov S., Wolf D., Totipotency, pluripotency and nuclear reprogramming; Adv. Biochem.  Eng. Biotechnol., 2009; 114: 185–9.

[11] Wang Z., Sarje A., Lin Che P., Yarema K.J.,      Moderate strength (0.23–0.28 T) static magnetic fields (SMF) modulate signaling and differentiation in human embryonic cells; BMC Genomics, 2009;  10: 356-365.

[12] Hsu S.H., Chang J.C., The static magnetic field accelerates the Osteogenic differentiation and mineralization of dental pulp cells; Cytotechnology, 2010; 62: 143–155.

[13] Teodori L., Albertini M.C., Rocchi M., Prsterà A., Fini M., Molinaro M., Adamo S., Static magnetic fields enhance skeletal muscle differentiation in vitro by improving myoblast alignment; Cytometry, 2007; 71(10): 846-856.

[14] Satija N.K., Singh V.K., Verma Y.K, Gupta P.,  Sharma S., Mesenchymal stem cell-based therapy: a new paradigm in regenerative medicine;  J. Cell. Mol. Med., 2007; 13(11-12): 4385-4402.

[15] Kumar R., Sharma A., Pattnaik A.K., Varadwaj P.K., Stem cells: An overview with respect to cardiovascular and renal disease; J.  Nat Sci. Biol. Med., 2010; 1(1): 43–52 .

[16] Maltman D.J., Hardy S.A., Przyborski S.A., Role of mesenchymal stem cells in neurogenesis and nervous system repairNeurochemistry International; 2011; 59: 347–356.

[17] Dong L., Huang L., Chen Y., Effects of Extremely Low Frequency  Magnetic Field on Growth, Kinetics, Metabolism and cell Cycle of Human Liver Cancer Cell; Bioelectromagnetism, 2005; 5: 1-6.

[18] Zhao G., Chen S., Wang L., Zhao Y., Cellular ATP Content, Was Decreased by a Homogeneous 8.5 T Static Magnetic Field Exposure:Roleof ReactiveOxygen Species; Bioelectromagnetics, 2011; 32: 94 -101.

[19] Hawley S.T., Hawley G.R., Flow Cytometry Protocols; Methods in Molecular Biology, 2011; 699:1-29.

[20] Dini L., Abbro L., Bioeffects of moderate-intensity static magnetic fields on cell cultures;  Micron, 2005; 36:195–217.

[21] Bekhite M.M., Figulla H.R., Sauer H., Wartenberg M., Static magnetic fields increase cardiomyocyte differentiation of Flk-1+ cells derived from mouse embryonic stem cells via Ca2+ influx and ROS production; Int. J. Cardiol., 2013; 167(3): 798-808.

[22] Zhang X., Liu X., Pan L., Lee I., Magnetic fields at extremely low-frequency (50 Hz, 0.8 mT) can induce the uptake of intracellular calcium levels in osteoblasts;  Biochemical and Biophysical Research Communications, 2010; 396: 662-666.

[23]  Cerella C., Cordiscoa S., Albertinib M.C.,  Accorsib A., et al.,  Magnetic fields promote a pro-survival non-capacitative Ca2+ entry via phospholipase C signaling; The International Journal of Biochemistry & Cell Biology, 2011; 43: 393–400.

[24] Piacentini R., et al., ELF=EMFs Promote Invitro Neurogenesis Via up Regulation of Cav-1 Channel Activity; J. Cell Physiol., 2007; 215: 129-139.

[25] Santella L., Ercolano E., Nusco G.A., The cell cycle: a new entry in the field of Ca2+ signaling CMLS; Cell. Mol. Life Sci., 2005; 62: 2405–2413.

[26] McCreary C.R., Dixon S.J., Fraher L.J., Carson J.L.,  et al., Real-time measurement of cytosolic free calcium concentration in Jurkat cells during ELF magnetic field exposure and evaluation of the role of cell cycle; Bioelectromagnetics, 2006; 27(5):  2354–2364.

[27] Maques M.M., Roman J., Ibanez M., et al., DNA Damage signsling in eukaryotes; Rev. Oncol., 2003;  5(3): 139-147.

[28] Flynn R.L., Zou L., ATR a master conductor of cellular responses to DNA replication stress; Trends in Biochemical Sciences March, 2011; 36(3): 133-140.

[29]  Hurley P.J., Wilsker D., Bunz F., Human cancer cells require ATR for cell cycle progression following exposure to ionizing radiation;  Oncogene, 2007; 26: 2535–2542.