نوع مقاله : مقاله کامل پژوهشی

نویسندگان

1 دانشجوی دکتری بیوفیزیک، گروه بیوفیزیک، دانشکده علوم زیستی، دانشگاه تربیت مدرس

2 دانشیار، گروه بیوفیزیک، دانشکده علوم زیستی، دانشگاه تربیت مدرس

3 دانشیار، گروه هماتولوژی، دانشکده پزشکی، دانشگاه تربیت مدرس

4 دانش‌آموختة کارشناسی ارشد، شرکت فناوری بن یاخته‌های رویان (بانک خون و بند ناف رویان)

5 دانش‌آموختة کارشناسی ارشد،گروه سلول‌های بنیادی و توسعة زیستی، مرکز تحقیقات علوم سلولی، پژوهشگاه رویان

10.22041/ijbme.2012.13103

چکیده

سلول‌های بنیادی مزانشیمی انسان که از بندناف نوزادان جدا و کشت داده شده بودند، به مدت 24 ساعت در معرض میدان مغناطیسی ایستا با شدت 24 میلی‌تسلا قرار گرفتند و درصد سلول‌های زنده و میزان پیشرفت در چرخة سلولی در نمونه‌های تابش دیده با شاهد مقایسه شد. نتایج نشان داد مجاورت با این میدان به مدت 24 ساعت در زمان‌های 36، 48 و60 ساعت پس از اعمال میدان سبب کاهش معنا‌داری در درصد سلول‌های زنده می‌شود. میزان پیشرفت این سلول‌ها در چرخه سلولی نیز، این یافته را تأیید کرد؛ اما بعد از گذشت 72 ساعت از زمان تابش‌دهی، این تغییر تا حد معناداری جبران شد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigating the impacts of Static Magnetic Field on cell viability and Cell Cycle Progression in Human Mesenchymal Stem cells

نویسندگان [English]

  • Maryam Sadri 1
  • Parviz Abdolmaleki 2
  • Saeed Abroun 3
  • Bahare Beiki 4
  • Fazel Samani 5

1 Ph.D Student, Biophysics Department, Faculty of Biological Sciences, Tarbiat Modares University

2 Associate professor, Biophysics Department, Faculty of Biological Sciences, Tarbiat Modares University

3 Associate professor, Hematology Department, Faculty of Medical Sciences, Tarbiat Modares University

4 M.Sc, Royan Stem Cell Technology Company (Royan Cord Blood Bank, R&D section

5 M.Sc, Department of stem cells and developmental biology, cell sciences research center, Royan Institute for Stem Cell Biology and Technology, ACECR

چکیده [English]

The Mesenchymal Stem cells derived from human newborn cords were cultured and exposed to a 24mT Static magnetic field for 24 hours. The viability percentage and the cell cycle progression was then investigated in exposed samples and the obtained results was compared with the control samples. The results clearly demonstrated a significant reduction of cell viability due to the exposure of 24 hours of SMF and post-exposure cultures within the time frames of 36,48,60 hours. The cell development through the cell-cycle, also verified this finding, however, 72 hours of post-exposure culture, significantly leveled off the drop in viable stem cell rates.

کلیدواژه‌ها [English]

  • Static magnetic fields
  • Mesenchymal stem cells
  • Cell cycle
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