نوع مقاله: مقاله کامل پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد،گروه زیست‌شناسی، دانشکده علوم، دانشگاه آزاد اسلامی واحد دامغان

2 دانشیار، گروه زیست‌شناسی، دانشکده علوم، دانشگاه آزاد اسلامی واحد دامغان

3 دانشیار، گروه بهداشت حرفه‌ای، دانشکده بهداشت، دانشگاه علوم پزشکی قزوین

10.22041/ijbme.2012.13105

چکیده

مواجهه مزمن با میدان‌های مغناطیسی می‌تواند آثار نامطلوبی بر حافظه و یادگیری داشته باشد. این مطالعه با هدف بررسی اثر حفاظتی عصاره زعفران بر اختلالات تثبیت حافظه در رت‌های در معرض تابش میدان مغناطیسی انجام شد. 120 موش صحرایی نر از نژاد ویستار در 12 گروه ده‌تایی به مدت 5 روز، هر روز به مدت 1، 3 و 5 ساعت در معرض تابش میدان مغناطیسی با شدت 5/2 تسلا قرار گرفتند و آثار حفاظتی عصاره زعفران با دوزهای 125 ،200 ، 300  میلی‌گرم برکیلوگرم در سطح معنی‌دار (05/0p) به روش یادگیری احترازی غیر فعال در دستگاه شاتل باکس در مقایسه با گروه کنترل بررسی شد. مواجهه یک ساعتی موش‌ها با میدان مغناطیسی تأثیر معنی‌داری بر تثبیت حافظه نداشت. افزایش زمان مواجهه به 3 و5 ساعت در مقایسه با گروه کنترل سبب اختلال درتثبیت حافظه شد(05/0P<). تجویز درون صفاقی عصاره زعفران در دوز 300  میلی‌گرم عصاره بر کیلوگرم تأثیر معنی‌داری در بهبود تثبیت حافظه در مقایسه با گروه کنترل داشت. تابش میدان مغناطیسی 5/2 میلی تسلا و50 هرتز سبب اختلال در تثبیت حافظه شد. عصاره آبی زعفران با دوز 300  میلی‌گرم بر کیلوگرم ممکن است دارای آثار حفاظتی و بهبود دهنده اختلالات تثبیت حافظه شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Protective effects of saffron extract against consolidation Impairment memory induced by magnetic field in rats

نویسندگان [English]

  • Samane Sedighi 1
  • Keyvan Keramati 2
  • Ali Safari-Varyani 3
  • Ahmad Nikpey 3

1 M.Sc., Department of Biology, Islamic Azad University of Damghan

2 Associate professor, Department of Biology, Islamic Azad University of Damghan

3 Associate professor, Department of Occupational Health, Qazvin University of Medical Sciences

چکیده [English]

Exposure to magnetic fields can effect on the learning and memory. The protective effect of saffron extract on memory consolidation disorders in rats exposed to magnetic fields was investigated. 120 male Wistar rats in 12 groups exposed to magnetic field For 5 days with intensity 2.5 tesla for 1, 3 and 5 hour and protective effects of saffron extract with doses of 125 mg, 200 mg and 300 mg (P ≤0.05) compared to the control group by passive avoidance learning method in shuttle box. One hour exposure with magnetic field had no effect on the rats’ memory consolidation (P ≤0.05). Increase exposure time to 3 and 5 hours had a memory consolidation Impairment compared to the control group (P ≤0.05). Administered rats with 300 mg Inter peritoneal saffron extract improved memory consolidation (P ≤0.05) compared to the control group. Exposure to magnetic fields 2.5 mT, 50 Hz impair memory consolidation. Saffron aqueous extract at a dose of 300 mg per kg may have a protective effect and be improvement consolidation impairments.

کلیدواژه‌ها [English]

  • Magnetic fields
  • Consolidation memory
  • Aqueous extract of saffron

[1] Berg-Beckhoff G., Kowall B., Breckenkamp J., Radio Frequency Electromagnetic Fields: Health Effects; Encyclopedia of Environmental Health, 2011: 721-727.

[2] Croiset G., Nijsen M.J., Role of corticotrophin- releasing factor, vasopressin and the autonomic nervous system in learning and memory; Eur J Pharmacl, 2000; 405(1): 225-234.

[3] Ruchi R., Learning and Memory, Biology; 1988; 202: 1-8.

[4] Janac B., Pesic V., Jelenkovic A., Vorobyov V., Prolic Z., Different effects of chronic exposure to ELF magnetic field on spontaneous and amphetamine-induced locomotor and stereotypic activities in rats; Brain Res Bull, 2005; 67:498-503.

[5] Jadidi M., Firoozabadi S.M., Rashidy-Pour A., Sajadi A.A., Sadeghi H., Taherian A.A., Acute exposure to a 50 Hz magnetic field impairs consolidation of spatial memory in rats; Neurobiol Learn Mem, 2007; 88: 387-392.

[6] Hendee W.R., The question of health effects from exposure to ELF; Health Physics, 1994; 66(2): 127-136.

 [7] Liu T., Wang S., He L., Ye K., Chronic exposure to low-intensity magnetic field improves acquisition and maintenance of memory; Neuroreport, 2008; 26: 549-552.

[8] Lyskov E.B., Chernyshev M.V., Mikhailov V.O., kozlov A.P., The effect of magnetic field with the frequency of 50Hz on behavior in rats depends on the value of the constant magnetic field; Biophysics, 1996; 41(4): 881-886.

[9] Sienkiewicz Z.J., Haylock R.G.E., Bartrum R., Saunders R.D., 50 Hz Magnetic Field Effects on the Performance of a Spatial Learning Task by mice; Bioelectromagnetics, 1998; 19: 486-493. 

 [10] Salzinger K., Behavioral effects of electromagnetic fields in animals. In: Carpenter DO, Ayrapetyan S, editors. biological Effects of electric and Magnetic Fields; New York: Academic, 1994; 1: 315-331.

 [11] Trimmel M., Schweiger E., Effects of an (50 Hz, 1 mT) electromagnetic field (EMF) on concentration in visual attention, perception and memory including effects of EMF sensitivity; Toxicol Lett, 1998; 97: 377-382.

 [12] Tarantilis P.A., Morjani H., Polissou M., Manafait M., Determination of saffron (Crocus sativus L.) components in crude plant extract using high performance liquid chromatography–UV–visible photodiode–array detection–mass spectrometry; J. Chromatogr, 1995; 699: 107–118.

 [13] Abdullaev F.I., Espinosa-Aguirre J.J., Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials; Cancer Detection and Prevention, 2004; 28: 426–432

[14] Asdaq S.M., Inamdar M.N., Potential of Crocus sativus (saffron) and its constituent, crocin, as hypolipidemic and antioxidant in rats; Appl. Biochem. Biotechnol, 2010; 162: 358–372.

[15] Rios J.L., Recio M.C., Giner R.M., Manez S., An update review of saffron and its active constituents; Phytother. Res., 1996; 10: 189–193.

[16] Abdullaev F.I., Espinosa-Aguirre J.J., Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials; Cancer Detection and Prevention, 2004; 28: 426–432.

[17] Abe K., Saito H., Effects of saffron extract and its constituent crocin on learning behaviour and long-term potentiation; Phytother. Res., 2000; 14: 149–152.

[18] Pitsikas N., Sakellaridis N., Crocus sativus L., extracts antagonize memory impairments in different behavioural tasks in the rat; Behav. Brain. Res., 2006; 173: 112–115.

[19] Zhang Y., ShoyamaY., Sugiura M., Saito H., Effects of Crocus sativus L. on the ethanol-induced impairment of passive avoidance performances in mice; Biol.Pharm. Bull., 1994; 17: 217–221.

[20] Ghadrdoost B., Vafaei A., Rashidy-Pour A., Hajisoltani R,Bandegi A.R., Motamedi   F., Haghighi S., Sameni H.R., Pahlvan S.,  Protective effects of saffron extract and its active constituent crocin against oxidative stress and spatial learning and memory deficits induced by chronic stress in rats; European Journal of Pharmacology, 2011; 667: 222–229.

[21] Babri S., Khalaji N., Effects of piracetam on memory disturbance due to elctromagnetic field exposure; Medical Journal of Ardebil University of Medical Sciences, 2005; 6(4): 357-362.

[22] Trimmel M., Schweiger E., Effects of ELF(50HZ, 1mT) electromagnetic field(EMF) on concentration in visual attention, perception and memory including effects of EMF sensitivity; Toxicol Lett, 1998; 96-97; 377-382.

[23] Jadidi M., Firoozabadi S.M., Taherian A.A, Sajadi A.A., The effect of 2 and 8 MT magnetic field on rat spatial memory consolidation; Feyz  Kashan University of Medical Sciences & Health Services, 2008; 12(1):15-20.

[24] Chadwick D., Cartlidge N., Bates D., Medical Neurology; Churchill Livingstone Press, 1989; chapter6: 128-150.

[25] Lee E.H., Liang K., lin W.R., Shiu W., Fluoxetine and 8-on-DPAT in the lateral septum enhances and impairs retention of an inhibitory avoidance response in Rats; Physiol. Behav., 1999; 51: 681- 688.

 [26] Kaviani Mogadam M., Firoozabadi S.M.P., Janahmadi M., Inhibitory effects of exposure to 50 Hz magnetic field in F1 neuronal soma membrane of Helix Aspersa; Biological Effects of Electromagnetic Fields, 3rd International Workshop, 2004; p. 4-8 October. Greece.

[27] Lai H., Carino M., 60 Hz magnetic fields and central cholinergic activity: effects of exposure intensity and duration; Bioelectromagnetics, 1999; 20: 284-289.

[28] Nikolskaya K.A., Shtemler V.M., Savonenko A.V., Weak magnetic fields and cognitive activity; Biofizica, 1996; 41(4): 897-903.

 [29] Akhondzadeh S.H., Fallah-Pour H., Afkham K.H., Jamshidi A.H., Khalighi-Cigaroudi F., Comparison of Crocus sativus L, and imoperamine in the treatment of mild to moderate depression: a pilot double-blind randomized trial; BMC Complement Altern Med, 2004; 4:12-6.

 [30] Ochiai T., Shimeno H., Protective effects of carotenoids from saffron on neuronal injury invitro andinvivo; Biochimica a Biophysica Acta, 2007; 1770: 578-584.

[31] Kazuho A., Hiroshi S., Effects of Saffron extract and its constituent crocin on learning behavior andlong-term potentiation; Phytotherapy Research, 2000; 14: 149-152.