نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 استادیار گروه بیوتکنولوژی کشاورزی، دانشکده علوم کشاورزی، دانشگاه شاهد
2 دانشیار گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس
3 دانشیار گروه ژنتیک، دانشکده علوم زیستی، دانشگاه تربیت مدرس
چکیده
در این تحقیق رشد سلولی، برخی پارامترهای فیزیولوژیک، تولید الکالوئید ضد سرطان تاکسول و بیان ژن در کشت سلولی فندق تحت اثر میدان مغناطیسی بررسی شد. سلولها در کشت تعلیقی با میدان مغناطیسی ایستا با شدت 30 میلی تسلا و در روزهای 8-11 بعد از واکشت، روزی 4 ساعت تیمار شدند. نتایج نشان داد میزان رشد و زنده‌مانی سلولها تحت اثر میدان قرار نگرفت؛ اما تولید H2O2و میزان پراکسیداسیون لیپیدهای غشایی افزایش یافت. فعالیت آنزیمهای فنیل آلانین آمونیا لیاز، پلی فنل اکسیداز و پراکسیداز تحت اعمال میدان مغناطیسی در مقایسه با شاهد افزایش یافت. تولید ترکیبات فنلی و تاکسول نیز در سلولهای تیمار شده در مقایسه با شاهد افزایش یافت. میدان مغناطیسی تاکسول درون سلولی را در مقایسه با تاکسول برون سلولی بیشتر افزایش داد و در کشتهای تیمار شده تولید تاکسول کل در مقایسه با کشتهای شاهد 9/2 برابر بود. بیان ژن 1- دئوکسی-D-زایلولوز -5- فسفات ردوکتوایزومراز نیز- که در تولید پیش‌سازهای تاکسول و بیوسنتز آن دخالت دارد- در سلولهای تیمار شده در مقایسه با شاهد افزایش یافت. به نظر می‌رسد میدان مغناطیسی با تحریک پاسخهای دفاعی سلول و القای بیان ژن دخیل در بیوسنتز تاکسول باعث افزایش تولید آن شده است.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
Stimulation of Taxol Production by Magnetic Field in Cell Culture of Hazel (CorylusavellanaL.)
نویسندگان [English]
- Ayatollah Rezaei 1
- Faeze Ghanati 2
- Mehrdad Behmanesh 3
1 Assistant Professor, Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, Shahed University
2 2Associate Professor, Department of Plant Biology, Faculty of Biological Science, TarbiatModares University
3 Associate Professor, Department of Genetics, Faculty of Biological Sciences, TarbiatModares University
چکیده [English]
In this study cell growth, some physiological parameters, production of Taxol and gene expression in cell culture of hazel under effect of the magnetic field were investigated. Cells in suspension culture were treated by a 30 mT static magnetic field on days 8-11 after subculture and 4 hours each day. The results showed that while the growth rate and viability of cells weren’t affected by the magnetic field but membrane lipid peroxidation rate and H2O2 production increased. Activity of phenylalanine ammonia lyase, polyphenol oxidase and peroxidase enzymes was increased by the magnetic field compared with control. Production of phenolic compounds and Taxolin treated cells showed an increase compared to those of control cells. Magnetic field increased intracellular Taxol more than extracellurTaxol, and in treated cultures total taxol production was 2.9-fold compared to control culture. Gene expression of 1- deoxy -D- xylulose -5 - phosphate reductoisomerase involved in producing Taxol precursors and in its biosynthesis was also increased in treated cells compared to control. It appears that magnetic field by stimulating cell defense responses and inducing gene expression involved in Taxol biosynthesis has resulted in improved its production.
کلیدواژه‌ها [English]
- Cell culture
- Hazel
- Taxol
- Magnetic field
- Gene expression
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