تاثیر میدان مغناطیسی ایستا با شدت 15 و 30 میلی تسلا بر روی چرخه سلولی در سلولهای مزانشیمی مغز استخوان رت القا شده با مواد تمایز دهنده سلولهای عصبی

نوع مقاله: مقاله کامل پژوهشی

نویسندگان

1 دانشجوی دکترای بیوفیزیک، گروه بیوفیزیک، دانشکده علوم زیستی، دانشگاه تربیت مدرس

2 دانشیارگروه بیوفیزیک، دانشکده علوم زیستی، دانشگاه تربیت مدرس

3 دانشیارگروه ژنتیک، دانشکده علوم زیستی، دانشگاه تربیت مدرس

10.22041/ijbme.2011.13144

چکیده

در این مقاله به تاثیر میدان مغناطیسی ایستا با شدتهای 15 و 30 میلی تسلا بر روی چرخه سلولی، سلولهای مزانشیمی مغز استخوان رت پرداخته شده است. سلولها بعد از کشت تحت تاثیر القاکننده های تمایز عصبی قرار گرفتند. القاء تمایز  و تابش دهی به مدت 2،4 و 6 ساعت صورت گرفت. یک گروه از سلولهای القا شده با مواد القاگر عصبی، تحت تابش میدان قرار گرفتند. در کنار این، گروه کنترل نیز وجود داشت که بدون تابش میدان بودند. برای تابش دهی از یک مولد میدان مغناطیسی ایستا که انکوباتوری در درون آن تعبیه شده بود استفاده گردید. بعد از تابش دهی، سلولها با اتانول 70 درصد تثبیت شدند. سپس با پروپیدیوم ایوداید رنگامیزی شده و توسط دستگاه فلوسایتومتر درصد فازهای G2/M,S,G1/G0  محاسبه گردید. برای محاسبه درصد مراحل مختلف چرخه سلولی از نرم افزار WinMdi2.9 استفاده شد. نتایج به صورت میانگین و انحراف معیار بدست آمد. نتایج نشان داد که میدان مغناطیسی با شدت 15 میلی تسلا تاثیری بر چرخه سلولی در سلولهای مزانشیمی رت در هیچ یک از زمانهای تابش دهی (4،2 و6 ساعت)  ندارد. میدان مغناطیسی ایستا با شدت 30 ملی تسلا و 2 ساعت تابش موجب افزایش فاز G2/M  گردید. تابش 4ساعته  30 میلی تسلا بر چرخه سلولی سلولهای مزانشیمی تاثیرگذار بوده و فاز S را بطور معنی دار کاهش داد.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

The effect of 15 and 30 militesla static magnetic field on the rat neuronal induced mesenchymal stem cell, cell cycle

نویسندگان [English]

  • Reza Masoomi Jahandizi 1
  • Parviz Abdolmaleki 2
  • Seyed Javad Mowla 3
1 Ph.D Student,Biophysics Department, Faculty of Biology Science, Tarbiat modares University
2 Associate Professor, Biophysics Department, Factulty of Biology Science, Tarbiat modares University
3 Associate Prodessor, Genetics Department, Factuly of Biology Science ,Tarbiat modares University
چکیده [English]

The effect of 15 and 30 mT of static magnetic field on the cell cycle of neural inductive rat BMSC was evaluated. The BMSC was inductived by neural inductive medium (NIM). Duration of inductive and Exposure time were 2, 4 and 6 hours. The cells induction to neural inductive medium associated with SMF as exposed group, besides we have a control group. The apparatus we used to generate the SMF was a locally designed SMF generator in which there was an incubator instrument capable to maintain the humidity, temperature and CO2 concentration in predefined level. After exposing, the cells were fixed, stained and their percent of cell cycle phases; G1, S, G2/M were elucidated using flowcytometer instrument. The WinMdi 2.9 Software was used to process data from flowcytometer and elucidation of phase's percents. The results showed SMF with 15 mT intensity did not significantly alter the cell cycle in three different (2, 4 and 6 hours) exposing times. Exposing 2 hours with 30 mT increased the G2/M phases in neural inductive BMSC comparing to the corresponding control. Static magnetic field decreased the percent of S phase in BMSC, during 4 hours exposure.

کلیدواژه‌ها [English]

  • Bone marrow stem cells
  • Rat
  • Static magnetic fields
  • Cell cycle
  • Flowcytometry
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