نوع مقاله : مقاله کامل پژوهشی

نویسندگان

1 دکترای بیومواد، عضو هیئت علمی پژوهشکده فناور یهای نو، دانشگاه صنعتی امیرکبیر

2 دکترای بیوشیمی، عضو هیئت علمی شرکت فنآوری بن یاخته

3 دکترای پلیمر، عضو هیئت علمی مرکز پژوهشی زیس تمواد، دانشگاه تهران

10.22041/ijbme.2010.13340

چکیده

داربست های مورد استفاده در مهندسی بافت باید علاوه بر عملکرد مناسب، متخلخل، زیست سازگار و زیست تخریب پذیر باشند. در این تحقیق، داربست های متخلخل کامپوزیتی PLGA/HA به روش تعویض حلال ساخته شده و با پلیمر سه قطعه ای روکش دهی و با نور UV استریل شدند. مشاهدات حاصل از میکروسکوپ الکترونی روبشی حاکی از تشکیل ریزساختار متخلخل با اندازه حفرات حدود50 mm  و حفرات به هم پیوسته است. سلول های بنیادی مزانشیم انسانی بر روی داربست ها بذرافشانی شدند و سلول ها در داخل این ساختار به طور مطلوب چسبیدند. رنگ آمیزی فلورسانس باDAPI نشان دهنده چسبندگی سلول های مزانشیم به نمونه های دارای روکش و نفوذ سلول ها به داخل حفرات بود. همچنین، به منظور بررسی میزان تکثیر سلول ها روی داربست ها، آزمایش MTT روی آنها انجام شد و نشان داد که تعداد سلول های کشت شده روی داربست ها در مقایسه با نمونه های کنترل تفاوت معناداری ندارد. از نتایج به دست آمده استنباط می شود که داربست های روکش دار شده با پلیمر سه قطعه ای بستر مناسبی برای سلول های مزانشیم و روش به کار رفته روشی کارامد در ساخت داربست مهندسی بافت استخوان است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Investigation of Human Mesenchymal Stem Cells Adhesion and Diffusion into Poly (lactic-co-glycolic acid)/Hydroxyapatite Porous Scaffold Coated with a Biodegradable Triblock Copolymer

نویسندگان [English]

  • Masoume Haghbin Nazarpak 1
  • Farzane Pourasgari 2
  • Mohammad Nabi Sarbolouki 3

1 PhD, New Technologies Research Center (NTRC), Amirkabir University of Technology

2 PhD, Stem Cells Technology Research Center

3 PhD, Biomaterials Research Center (BRC), University of Tehran

چکیده [English]

The scaffolds for bone tissue engineering should consider the functional requirements: porosity, biocompatibility, and biodegradability. In this study, porous Poly (lactic-co-glycolic acid)/Hydroxyapatite composites were prepared with different weight ratios. Porous samples were fabricated by freeze-extraction method, coated with triblock copolymer and sterilized by UV. Then, human mesenchymal stem cells were cultured on scaffolds. Microstructural studies with SEM suggest the formation of about 50 micrometer size porous structure and interconnected porosity so that cells adhesion within the structure is well in depth in coated samples. DAPI fluorescence microscopy showed cells adhesion to the coated scaffolds and cells diffusion into the pores. Also, direct assay of cell proliferation performed with MTT test showed that, cells grew on the scaffold similar to or more than control samples result. Therefore, these findings suggest that the triblock-coated Poly (lactic-coglycolic acid)/ Hydroxyapatite porous composite scaffolds could provide cells adhesion and proliferation and are appropriate matrices for bone tissue engineering.

کلیدواژه‌ها [English]

  • Scaffold
  • Bone tissue engineering
  • Freeze-extraction
  • Mesenchymal stem cells
  • Cell adhesion
  • Porosity
  • PLGA
  • Composite
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