Effect of Static Stretch on Alignment of Human Mesenchymal Stem Cells

Rabbani, Mohsen; Tafazzoli Shadpour, Mohammad; Goli Malekabadi, Zahra; Janmaleki, Mohsen; Khorasani, Mohammad Taghi; Shokrgozar, Mohammad Ali

doi:10.22041/ijbme.2010.13343

Effect of Static Stretch on Alignment of Human Mesenchymal Stem Cells

Document Type : Full Research Paper

Authors

Mohsen Rabbani ¹

Mohammad Tafazzoli Shadpour ²

Zahra Goli Malekabadi ³

Mohsen Janmaleki ⁴

Mohammad Taghi Khorasani ⁵

Mohammad Ali Shokrgozar ⁶

¹ PhD Candidate, Biomechanics Group, School of Biomedical Engineering, Amirkabir University of Technology

² Associate Professor, Biomechanics Group, School of Biomedical Engineering, Amir Kabir University of Technology

³ M.Sc Student, Biomechanics Group, School of Biomedical Engineering, Amir Kabir University of Technology

⁴ Research Scientist, Nano Medicine & Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences

⁵ Associate Professor, Iran Polymer and Petrochemical Research Institute

⁶ Associate Professor, National Cell Bank, Pasteur Institute of Iran

https://doi.org/10.22041/ijbme.2010.13343

Abstract

Vital function of the cell is correlated with the mechanical loads that the cell experiences. The cell shape and morphology are also related to its mechanical environments. Different methods have been proposed to obtain cell groups with the same morphology and alignment which considered desirable features in tissue engineering applications. For instance, applying cyclic loading makes cells elongated and aligned as bundles in a specific direction to the tension axis. Applying static stretches also affect the cells morphology, extra-cellular matrix, enzymes secretion and genes expression. The effect of applying in vivo static stretch on cellular alignment was evaluated in this study. Human mesenchymal stem cells (hMSCs) were cultured on the elastic membrane, and then subjected to static stretch. The results demonstrated that applying a 10% static stretch for 24 hours aligns intra-structure actin filaments and applying a 20% static stretch had a significant effect on the arrangement of the oriented fibers.

Keywords

Static stretch

Cellular loading

Cellular alignment

Human Mesenchymal stem cell

Tissue engineering

Subjects

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