بررسی خواص فیزیکی، شیمیایی و پاسخ های سلولی بیوسرامیک ژیپس حاوی غلظت های مختلف استرانسیم

نوع مقاله: مقاله کامل پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، پژوهشکده سرامیک، پژوهشگاه مواد و انرژی

2 استادیار، گروه فناوری، پژوهشکده سرامیک، پژوهشگاه مواد و انرژی

10.22041/ijbme.2009.13390

چکیده

هدف این مقاله بررسی اثر وارد کردن مقادیر مختلف یون استرانسیم (با درصد وزنی 0.19-2.23) به درون شبکه بیوسرامیک سولفات کلسیم بر خواص فیزیکی، ساختاری و زیستی در محیط in vitro و مقایسه آن با خواص نمونه سولفات کلسیم دی هیدراته (ژیپس) خالص به عنوان نمونه شاهد است. ژیپس حاوی استرانسیم (Sr-ژیپس) از مخلوط کردن پودر سولفات کلسیم نیمه هیدراته و محلول نیترات استرانسیم حاصل شد، سپس نمونه ها با آب مقطر شستشو داده شدند تا نمک های باقیمانده خارج شود. ژیپس تنها فازی بود که در ترکیب نمونه ژیپس خالص و استرانسیم دار حاصل شد و همچنین یک جابه جایی در زاویه پراش اشعه X به سمت زوایای کوتاه تر در الگوهای پراش نمونه های حاوی استرانسیم مشاهده شد. حضور استرانسیم در ساختار ژیپس استحکام ژیپس را افزایش و حلالیتش را کاهش داد. ریزساختار تمامی نمونه ها شامل بلورهای کوچک میله ای شکل و درهم فرو رفته است و در این میان بلورهای Sr-ژیپس طویل تر و ضخیم ترند. الگویEDXA نمونه ژیپس حاوی استرانسیم حضور یون های کلسیم و سولفور به عنوان عناصر اصلی ژیپس و مقدار جزئی استرانسیم را نشان داد. رهایش پیوسته استرانسیم از نمونه ژیپس حاوی استرانسیم به درون محلول شبیه سازی شده با بدن به مدت 14 روز مشاهده شد. نرخ تکثیر و فعالیت آلکالین فسفات سلول های استخوان ساز کشت داده شده روی نمونه های حاوی Sr نسبت به ژیپس خالص بهتر بود.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Physicochemical Properties And Cellular Responses Of Various Amounts Of Strontium-Doped Gypsum Bio Ceramics

نویسندگان [English]

  • Hadis Bandegani 1
  • Saeed Hesaraki 2
  • Masoud Alizadeh 2
1 M.Sc Student of Materials Engineering, Ceramics Department, Materials and Energy Research Center
2 Assistant Professor, Ceramics Department, Materials and Energy Research Center
چکیده [English]

The aim of the present paper is to investigate the effect of incorporating various amounts of strontium ions (0.19 - 2.23 wt%) into calcium sulfate bio ceramics on the physical, structural properties and in vitro bioactivity and compare these properties with those of a pure calcium sulfate dehydrate (gypsum) as control. Strontium-doped gypsum (Sr-gypsum) was obtained by mixing calcium sulfate hemihydrates powder and solutions of strontium nitrate followed by washing the specimens with distilled water for the removal of residual salts. Gypsum was the only phase found in the composition of both pure and Sr-gypsum meanwhile a shift into lower diffraction angles was observed in the x-ray diffraction patterns of doped specimens. The Sr-doped sampled exhibited higher compressive strength and lower solubility than pure gypsum. Microstructure of all gypsum specimens had been composed of many rod-like small crystals entangled to each others with more elongation and higher thickness in the cases of Sr-gypsum. EDXA pattern of Sr-gypsum showed the presence of calcium and sulfur ions as the main elements of gypsum as well as slight amount of strontium ion. A continuous release of strontium was observed from the Sr-gypsum after soaking in simulated body fluid for 14 days. Proliferation rate of cultured osteoblasts and higher alkaline phosphatase activity on doped samples was better compared to pure gypsum.

کلیدواژه‌ها [English]

  • Strontium
  • Bio Ceramics
  • In vitro
  • Gypsum
  • Sr-Containing Calcium Sulfate
  • Osteoblasts Proliferation
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