نوع مقاله: مقاله کامل پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد مهندسی پزشکی، دانشکده مهندسی پزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران

2 دانشیار، گروه بیومواد، پژوهشکده فناوری نانو و مواد پیش‌رفته، پژوهشگاه مواد و انرژی، کرج

3 استادیار، گروه مواد دندانی، دانشکده دندان‌پزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران

10.22041/ijbme.2014.14709

چکیده

داربست­های سه­بعدی که شباهت ریزساختاری بسیاری به ماتریکس خارج سلولی (ECM)داشته و از جنس کوپلیمر لاکتیک گلیکولیک­اسید (PLGA)/ژلاتین هستند به روش ریخته­گری انجمادی تهیه شدند. با این روش، امکان انجماد جهت­دار محلول پلیمری مهیّا شده و اثرهای مطلوب آن بر خواص فیزیکی/مکانیکی داربست­ها مورد ارزیابی قرار گرفت. برای انحلال دو پلیمر PLGA و ژلاتین از استیک­اسید به عنوان حلال مشترک استفاده شد. تصاویر به دست آمده از میکروسکوپ الکترونی روبشی (SEM) نشان­گر دست­یابی به داربست­هایی با تخلخل­های باز، درصد تخلخل بیش­از 95 درصد و با توزیع ابعادی حدود 400-50 میکرومتر در مقطع عمود بر جهت انجماد و 300-50 میکرومتر در مقطع موازی با جهت انجماد هستند. نتایج تخلخل­سنجی جیوه­ای، توزیع تخلخل 200-100 میکرومتر را نشان داد. نتایج طیف­سنجی فروسرخ (FTIR) حاکی­از عدم تغییر ساختاری مواد پس­از ساخت داربست­ها است. آزمون استحکام فشاری (MPa 2/3) نشان داد که داربست­های ساخته شده از استحکام مناسبی برخوردار هستند. نتایج آزمون جذب آب (950%) و زیست تخریب پذیری داربست­ها، گویای حفظ پایداری ساختار و تبادل­های سلولی درطی دوره­ی تخریب است. نتایج نشان می­دهد که داربست­های مذکور ویژگی­های اولیه و خواص مطلوبی برای استفاده در مهندسی بافت دارند و گزینه­ی مناسبی برای حمایت از چسبندگی سلولی و حفظ پایداری ساختاری در بازه­ی زمانی مورد نظر هستند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Morphological and Physiochemical Characteristics of biodegradable PLGA-Gelatin Scaffolds by Unidirectional Freezing Technique

نویسندگان [English]

  • Farnaz Ghorbani 1
  • Ali Zamanian 2
  • Hanie Noje Dehian 3

1 M. Sc Student, Department of Biomedical Engineering, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Associate Professor, Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Karaj, Iran

3 Assistant Professor, Department of Dental Materials, Dental School, Shahid Beheshti University of Medical Science, Tehran, Iran

چکیده [English]

In this study, we fabricated 3-dimentional PLGA-gelatin scaffolds with aligned-oriented pores by freeze casting technique which is similar to Extra Cellular Matrix (ECM), and evaluated its effect on both physical and mechanical features. Dissolving synthetic (PLGA) and natural (Gelatin) polymers in common solvent was one of the strengths of this investigation. Scanning electron microscopy (SEM) micrographs indicated that scaffolds contained 95% interconnected pores with diameter about 50-400 µm in horizontal direction and 50-200 µm in vertical direction. Moreover, the results of mercury intrusion porosimetry represented diameter of pores in range of 100–300 µm. According to fourieres transform infrared (FTIR) spectrum there was no inappropriate interactions during processing. Additionally, mechanical analysis (3.2 MPa) of PLGA-gelatin constructs illustrated that polymeric scaffolds can withstand mechanical loads in freezing direction. Based on the water absorption (950%) and biodegradation results, samples can support cellular interactions and prevent their integrity during tissue regeneration. In brief, freeze casted PLGA-gelatin scaffolds can provide unidirectional matrix with desired physical and mechanical characters to regenerate lesions.

کلیدواژه‌ها [English]

  • Lactic-co-Glycolic acid
  • Gelatin
  • freeze casting
  • polymeric scaffolds
  • Acetic acid

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