Nanobiotechnology / Bionanotechnology / Nanobiology
Mohadese Shahriaripour; Sasan Asiaei
Volume 15, Issue 1 , May 2021, , Pages 87-97
Abstract
Cerium oxide nanoparticles have many applications in medicine. Particle size, shape and concentration of nanoceria are very important for biological applications and biocompatibility. The synthesis method of cerium oxide nanoparticles has an important role in determining nanoceria shape, particle size ...
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Cerium oxide nanoparticles have many applications in medicine. Particle size, shape and concentration of nanoceria are very important for biological applications and biocompatibility. The synthesis method of cerium oxide nanoparticles has an important role in determining nanoceria shape, particle size and concentration. In this project, the effective parameters in determining the concentration, size and size distribution, crystallinity and production of maximum cerium oxide produced from the reactants were simulated and tested. Since in different method, particle size control has become an important challenge, microfluidic chips were used to control particle size. Among the existing methods for nanoparticle synthesis, co-precipitation method was chosen because of its simplicity, cheapness and short time method compared with other methods. Cerium nitrate and sodium hydroxide were used as raw materials to synthesize cerium oxide nanoparticles. Simulations were performed in Comsol and then the results were used for experimental tests, comparison and validation. The nanoparticles were characterized for size and size distribution using x-ray diffraction. The results of this study showed that the use of microfluidic chips is an effective method for controling nanoparticle size. Increasing concentration of sodium hydroxide can complete reaction and have maximum efficiency and decreasing the reactives velocity can reduce the size dispersion, increases the crystallinity and particle size. The yellow precipitate produced, according to Scherer equation, contains cerium oxide nanoparticles with particle size of 1.16±0.1 nm and 85% of crystallinity.
Nanobiotechnology / Bionanotechnology / Nanobiology
Yousef Habibi Sooha; Mohadese Mozafari; Moharam Habibnejad Korayem
Volume 11, Issue 3 , September 2017, , Pages 231-242
Abstract
In the most contact theories such as Hertz, DMT and JKR, which are the most practical contacts models, biological particles are considered as a spherical elastic particle, which is not the best assumption. In this assumption, the history of loadings are not considered in that the history of strains ...
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In the most contact theories such as Hertz, DMT and JKR, which are the most practical contacts models, biological particles are considered as a spherical elastic particle, which is not the best assumption. In this assumption, the history of loadings are not considered in that the history of strains and stresses will not analyzed properly. Therefore, in the first part of this paper, three models of elastic in spherical geometry have been developed to the viscoelastic models. By simulations and comparing the results with the experimental data of MCF-10A (breast-cancer cell), which is derived by Atomic Force Microscopy, it is revealed that viscoelastic models are more accurate than elastic models in the force-indentation curves. Then, according to the fact that most bacteria's geometry is cylindrical, contact theory for a sphere and cylinder have been developed and simulated for three groups of nanobacteria (Epidermidis, SallyVirus, and Aureus). By comparing simulations results with experimental data we observe that elastic models are not reasonable and contacts radius in viscoelastic model are smaller than they were for elastic models.
Nanobiotechnology / Bionanotechnology / Nanobiology
Mah Monir Karimzade; Ladan Rashidi; Fariba Ganji; Mitra Ahmadi; Sattar Tahmasebi Enferadi
Volume 8, Issue 4 , February 2015, , Pages 385-398
Abstract
The aim of this research is the preparation of a system based on mesoporous silica nanoparticles (MSN) for delivery of Rivastigmine hydrogen tartrate and investigating of the system cytotoxicity, with or without drugs, on the human brain neuroblastoma cells (SY5Y). Rivastigmine is a hydrophilic and a ...
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The aim of this research is the preparation of a system based on mesoporous silica nanoparticles (MSN) for delivery of Rivastigmine hydrogen tartrate and investigating of the system cytotoxicity, with or without drugs, on the human brain neuroblastoma cells (SY5Y). Rivastigmine is a hydrophilic and a hydrophobic drug which is used for treatment of Alzimerʾs disease. In this study MSN were synthesized and characterized by scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, x-ray diffraction, N2 adsorption isotherms, and z-potential analysis. Results showed that all MSN were spherical with the same structure. The mean size of nanoparticles was 100±13 nm and the mean diameter of pores was 2.15 nm. The loading capacity and efficiency of rivastigmine hydrogen tartrate were obtained 20.88, and 25%, respectively. Release of rivastigmine from nanoparticles in the simulated gastric and body fluid during 24 h were obtained 70.5 and 79.6%, respectively, which was shown the slightly fast release of rivastigmine in simulated gastric fluid. The cytotoxicity effect of nanoparticles with and without rivastigmine was done by MTT assay on SY5Y cell lines. Results showed that the in vitro rivastigmine release from the nanoparticles containing of it exhibited the more treatment property as free rivastigmine on SY5Y.
