Iranian Journal of Biomedical Engineering (IJBME)
نشریه علمی مهندسی پزشکی زیستی

Modelling of Sandwich-Type Amperometric Micro-Biosensor with Perforated Membrane

Document Type : Full Research Paper

Authors

Aminreza Noghrehabadi 1
Mohammad Hosein Heidarshenas 2
Reza Bahoosh 3

1 Associate Professor, Department of Mechanical Engineering, Engineering Faculty, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 MS.D Student, Department of Mechanical Engineering, Engineering Faculty, Shahid Chamran University of Ahvaz, Ahvaz, Iran

3 Assistant Professor, Department of Mechanical Engineering, Engineering Faculty, Shahid Chamran University of Ahvaz, Ahvaz, Iran

https://doi.org/10.22041/ijbme.2017.55758.1175
Abstract
A two-dimensional-in-space mathematical model of amperometric micro biosensors with selective and perforated membranes has been proposed and analyzed. The model involves the geometry of micro or nano meter holes partially or fully filled with an enzyme. The model is based on a system of the reaction-diffusion equations containing a nonlinear term related to the Michaelis-Menten enzymatic reaction. In this study, in order to generate general equation, first, dimensionless parameters are introduced and then by replacing them into governing equation are converted to dimensionless equations.The general equations have been solved numerically in 2D space.. Using numerical simulation of the biosensor action, the influence of the geometry of the holes as well as of the filling level of the enzyme in the holes on the biosensor response was investigated. For this purpose three different geometries including cylindrical, upright circular and downright circular cone for cavities are considered and the impact of these geometries on the response of the biosensor in different levels of enzyme are obtained. Biosensor's respond based on rate of enzyme level variations to slope of the cone variations are determined. In the biosensor, as the level of enzyme rises in all three geometries, the biosensor output current increases. Under the same conditions, the sensitivity of biosensor in upright circular cone is more than the other two geometries and increases with a decrease in conical gradient. As long as the enzymatic properties are the same, the more biosensor's number, the more sensitivity.Moreover, a concept known as reduced dimensionless current is introduced by providing and calculating dimensionless current in the biosensor.

Keywords

Modelling
Simulation
Biosensor
Dimensionless reduced current

Subjects

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Iranian Journal of Biomedical Engineering (IJBME)
Volume 10, Issue 2
Summer 2016
Pages 123-136

  • Receive Date 31 December 2016
  • Revise Date 06 August 2017
  • Accept Date 25 August 2017

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