Numerical analysis of a single cultured mesenchymal stem cell under pressure stimulation using atomic force microscopy and finite element method

Alihemmati, Zakieh; Vahidi, Bahman; Haghighipour, Nooshin

doi:10.22041/ijbme.2014.13045

Document Type : Full Research Paper

Authors

¹ MSc of Biomedical Engineering-Tissue Engineering, Faculty of New Sciences and Technologies (FNST), University of Tehran, Tehran, Iran

² Assistant Professor of Biomedical Engineering, Faculty of New Sciences and Technologies (FNST), University of Tehran, Tehran, Iran

³ National cell bank of Iran, Pasteur Institute of Iran, Tehran, Iran

10.22041/ijbme.2014.13045

Abstract

Body cells, including mesenchymal stem cells are subject to a lot of mechanical forces. The type and magnitude of these forces are different in different physiological and pathological conditions. They cause a wide variety of cell responses and are able to change metabolisms and functions of the cell. Analysis of stem cell response to mechanical stimulation is very important in recognizing healthy and diseased condition of tissues and cells. Differentiation potential of mesenchymal stem cells to specialized cells makes them important cell sources in tissue engineering. In this study, atomic force microscopy and finite element method and used mechanical effects on a stem cellaresimulated which includes cell behavior due to strain andstress distributions in internal components of the cell. In this study, the ADINA software used to simulate mechanical behavior of the cell components (cell membrane, cytoplasm and nucleus) under a compressiveload. Results indicate mechanical response of stem cells in the body through which they can differentiate into bone cells and cartilage under compressive loads in the physiological range. This study has some considerable innovations as compared with the similar studies in the literature which is because of the kind of cells has been used (adipose-derived stem cells) as well as and also using precise material models for cell components based on the data extracted from laboratory tests for mechanical properties of the cell. Furthermore, this study can be considered as an important initial step for future studies on different patho-cells and analyzing their responses to mechanical loading using a similar method of this study to find new diagnostic methods. Also, it can be used to deepen pathological studies of the cells and the tissues.

Keywords

Main Subjects

References

[1] D. Aubry, H. Thiam, M. Piel, R. Allena, “A computational mechanics approach to assess the link between cell morphology and forces during confined migration” Journal of Biomech Model Mechanobiol 2014.

[2] C. Borau, R. Kamm, “A time-dependentphenomenological model for cell mechano-sensing” Journal of Biomech Model Mechanobiol 13, 451–462, 2014.

[3] S. Wong, S. Teo, S. Park, K. Chiam, E. Yim, “Anisotropic rigidity sensing on grating topography directs human mesenchymal stem cell elongation” Journal of Biomech Model Mechanobiol 13, 27–39, 2014.

[4] E. Canovi´c, et al. “Biomechanical imaging of cell stiffness and prestress with subcellular resolution” Journal of Biomech Model Mechanobiol 13, 665–678, 2014.

[5] T. Woolley, et al. “Cellular blebs: pressure-driven, axisymmetric, membrane protrusions” Journal of Biomech Model Mechanobiol 13, 463–476, 2014.

[6] C. Obbink-Huizer, et al. “Computational model predicts cell orientation in response to a range of mechanical stimuli” Journal of Biomech Model Mechanobiol 13, 227–236, 2014.

[7] S. Verbruggen, et al. “Fluid flow in the osteocyte mechanical environment: a fluid–structure interaction approach” Journal of Biomech Model Mechanobiol 13, 85–97, 2014.

[8] C. Giverso, et al. “Influence of nucleus deformability on cell entry into cylindrical structures” Journal of Biomech Model Mechanobiol 13, 481–502, 2014.

[9] R. Katzengold, et al. “Simulating single cell experiments in mechanical testing of adipocytes” Journal of Biomech Model Mechanobiol 2014.

[10] M. R. K. Mofrad, R. D. Kamm, “Cytoskeletal mechanics models and measurements”2006.

[11] K. B. Bernick, “Cell Biomechanics of Central Nervous system” Massachusetts Institute of Technology 2011.

[12] D. Rafael, V. González-Cruz, C. Fonseca, M. Eric, “Darling Cellular mechanical prop differentiation potential mesenchymal stem cells” Journal of Proceedings of the National Academy of Sciences 109, 1523- 1529, 2012.

[13] N. Slomka, A. Gefen, “Confocal microscopy-based three-dimensional cell-specific modeling for large deformation analyses in cellular mechanics” Journal of Biomechanics 43, 1806–1816, 2010.

[14] F. Guilak, R. John, s.l.Tedrow, R. Burgkart, “Viscoelastic Properties of the Cell Nucleus” Journal of Biochemical and Biophysical Research Communications 269, 781–786, 2000.

[15] H. Hatami, M. Marbini, R. K. Mofrad, “Cytoskeletal Mechanics and Cellular Mechanotransduction: a molecular prospective” book auth. 2010.

[16] A. C. Guyton, J. E. Hall, “Text book of medical physiology” Elsevier Science 2005.

[17] S. A. Berger, L. D. Jou, “Flows in stenotic vessels” Journal of Annual Review of Fluid 32 347-382, 2000.

[18] A. Gefen, “cellular and biomolecular mechanics and mechanobiology” springer, 2011.

[19] W. Christopher Harland, J. Miranda Bradley, R. Parthasarathy. “Phospholipid bilayers are viscoelastic” Journal of Proceedings of the National Academy of Sciences (PNAS) 107, 19146–19150, 2010.

[20] Suncica Canic. “Fluid-Structure Interaction In Blood Flow. S.L. : Women In Mathematics” 2006.

[21] O. C. Zienkiewicz, R. L. Taylor, J.Z. Zhu, “The Finite Element Method: Its Basis and Fundamentals” Sixth edition. Oxford OX2 8D 2005.

[22] E. Leopold, A. Gefen, “A simple stochastic model to explain the sigmoid nature of the strain-time cellular tolerance curve” Journal of Tissue Viability 21, 27-36, 2012.

[23] L. Zheng, et al. “A Porous Elastic Model for Bacterial Biofilms:Application to the Simulation of Deformation of Bacterial Biofilms Under Microfluidic Jet Impingement” Journal of Biomechanical Engineering 134, 051003, 2012.

[24] A. Kweku, “Measurement Techniques for Cellular Biomechanics In Vitro” Journal of Experimental Biology and Medicine 233, 792-809, 2008.

[25] S. Shojaei, et al. “Essential Functionality of Endometrial and Adipose Stem Cells in Normal and Mechanically Motivated Conditions” Journal of Biomaterial and Tissue Engeeniring 3, 581-588, 2013.

[26] A. Aryaei, A. Jayasuriya “Mechanical properties of human amniotic fluid stem cells using nanoindentation” Journal of Biomechanics 46, 1524–1530, 2013.

[27] N. Slomka, S. Or-Tzadikario, D. Sassun, A. Gefen, “Membrane-stretch-induced cell death in deep tissue injury: computer model studies” Journal of Cellular and Molecular Bioengineering 2, 118–132, 2009.

[28] M. Robin, Delaine-Smith et al. “Mesenchymal stem cell responses to mechanical stimuli” Journal of Muscles, Ligaments and Tendons 2, 169-180, 2012.

[29] S. Or-Tzadikario, A. Gefen, “Confocal-based cell-specific finite element modeling extended to study variable cell shapes and intracellular structures: the example of the adipocyte” Journal of Biomechanics 44, 567–573, 2011.

Iranian Journal of Biomedical Engineering

Numerical analysis of a single cultured mesenchymal stem cell under pressure stimulation using atomic force microscopy and finite element method

References

References

Volume 8, Issue 2 - Serial Number 2
June 2014
Pages 135-149

Numerical analysis of a single cultured mesenchymal stem cell under pressure stimulation using atomic force microscopy and finite element method

References

References

Volume 8, Issue 2 - Serial Number 2June 2014Pages 135-149

Volume 8, Issue 2 - Serial Number 2
June 2014
Pages 135-149