The Effect of Collagen on the Synthesis of Calcium Phosphate Nanoparticles

Salemi, Hoda; Behnamghader, Ali Asghar; Baghaban Eslaminejad, Mohammad Reza; Ataei, Mohammad

doi:10.22041/ijbme.2013.13120

Document Type : Full Research Paper

Authors

¹ Ph.D student, Biomaterials Department, Faculty of Biomedical Engineering, Science & Research Branch, Islamic Azad University(IAU), Department of biomedical engineering

² Assistant Professor, Biomaterials group, Materials and Energy Research Center

³ Professor , Department of Stem Cell and Developmental Biology , at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR

⁴ Associate Professor, Polymer Petrochemical Institute

10.22041/ijbme.2013.13120

Abstract

Collagen and Hydroxyapatite (HA) nanoparticles are significant constituent of the natural bone. In this study, the effect of collagen on the morphological and phase characteristics of calcium phosphate nanoparticles was investigated. The synthesis reaction was initiated by mixing H₃PO₄ as phosphorous source and CaCl₂ as calcium source in presence of Collagen Type 1. Collagen concentration in suspension and Ca to P ratio was 1% and 1.67 respectively. The morphology and structure of samples (with collagen and without collagen), heat treated at 600 ⁰C were characterized by X-Ray diffraction (XRD), Fourier transformation infrared (FTIR) and Scanning electron microscopy (SEM). More fine and flake-like shape particles were observed in the SEM images of sample synthesized in the presence of collagen compared to the control sample which was constituted of larger granular particles. The XRD results revealed the powders were composed of hydroxyapatite and octacalcium phosphate and the sample synthesized in the presence of collagen was less crystalline. The amide bands of collagen and P-O and OH characteristic peaks were identified in FT-IR spectra.

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Iranian Journal of Biomedical Engineering

The Effect of Collagen on the Synthesis of Calcium Phosphate Nanoparticles

References

References

Volume 6, Issue 4 - Serial Number 4
June 2012
Pages 249-255

The Effect of Collagen on the Synthesis of Calcium Phosphate Nanoparticles

References

References

Volume 6, Issue 4 - Serial Number 4June 2012Pages 249-255

Volume 6, Issue 4 - Serial Number 4
June 2012
Pages 249-255