Document Type : Full Research Paper


1Assistant Professor, Reproductive Biotechnology Research Center of Ebne Sina, Medical Research Institute of New Technology,



Silk fibroin is fibrous proteins with excellent mechanical properties which are produced by wide group of animals such as Bombyx Mori. Silk fibroin with specific molecular structure can be processed into a diverse set of morphologies. Additionally, biotechnologically produced silk proteins will allow the preparation of a new generation of protein-based bio-polymeric materials with programmed properties for a wide variety of exciting medical applications. In this study, silk protein was extracted from Bombyx Mori’s cocoons and evaluated by FTIR and XRD methods. Results showed sharp amide peaks in 1655 cm-1 and 1530 cm-1 wavelength in FTIR spectrum pattern confirming existence of fibroin. SEM images of the fibers showed continuous fibers with cross-section between 14 to 24 μm. Biocompatibility tests were carried out through seeding osteoblasts cell line G292 on 2D film as well as fibers. Adhesion and proliferation of osteoblasts were investigated by MTT assay which showed no cytotoxicity. Therefore, fibroin appears to be remarkable material for prospect application in biomedicine.


[1]     D. Chow, M.L. Nunalee, D.W. Lim, A. J. Simnick, A. Chilkoti, “Peptide-based biopolymers in biomedicine and biotechnology”, Materials Science and Engineering (2008), R 62, 125–155.
[2]     J.G. Hardy, L.M. Romer, T.R. Scheibel, “Polymeric materials based on silk proteins”, Polymer (2008), 49, 4309–4327.
[3]     C. Veparia, D.L. Kaplan,” Silk as a biomaterial”, Prog. Polym. Sci. (2007), 32, 991–1007.
[4]     O. Hakimi, D.P. Knight, F. Vollrath, P. Vadgama,” Spider and mulberry silkworm silks as compatible biomaterials”, Composites: Part B (2007), 38, 324–337.
[5]     M. Yang, J. Kawamura, Z. Zhu, K. Yamauchi, T. Asakura, “Development of silk-like materials based on Bombyx mori and Nephila clavipes dragline silk fibroins”, Polymer (2009), 50, 117–124.
[6]     S.C. Kundua, B.C. Dasha, R. Dasha, D. L. Kaplan,” Natural protective glue protein, sericin bioengineered by silkworms: Potential for biomedical and biotechnological applications”, Progress in Polymer Science (2008), 33, 998–1012.
[7]     A. Sonthisombat, P.T. Speakman,” Silk: queen of fibers”, Thesis, University of leeds, UK, 2003.
[8]     K. Numata, D. L. Kaplan,” Differences in Cytotoxicity of _-Sheet Peptides Originated from Silk and Amyloid _”, Macromolecular Bioscience (2011), 11, 60–64.
[9]     Y. Zhang, “Applications of natural silk protein sericin in biomaterials”, Biotechnology Advances (2002), 20, 91– 100.
[10] E.S. Sashina, A. M. Bochek, N. P. Novoselov, D.A. Kirichenko, “Structure and Solubility of Natural Silk Fibroin”, Russian Journal of Applied Chemistry (2006), 79, 869–876.
[11] G. Vunjak-Novakovic, R.I. Freshney, “Culture of cells for tissue engineering”, New Jersey, John Wiley & Sons, (2006), 323–330.
[12] S. Louisia, M. Stromboni, A. Meunier, L. Sedel, H. Petite,”Repair of a large segmental diaphyseal defect with coral: Influence of fresh bone marrow on osteogenesis and coral resorption" J. of bone and Joint Surgery (1999), 81, 719–724.
[13] U. Kim, J. Parka, H. J. Kim, M. Wadac, D. L. Kaplan,” Three-dimensional aqueous-derived biomaterial scaffolds from silk fibroin”, Biomaterials (2005), 26, 2775–2785.
[14] G. H. Altman, F. Diaz, C. Jakuba, T.Calabro, R. L. Horan, J. Chen, H. Lu, J. Richmond, D.L. Kaplan,” Silk-based biomaterials”, Biomaterials (2003), 24, 401–416.
[15] J.G. Hardy, T.R. Scheibel, “Composite materials based on silk proteins”, Progress in Polymer Science (2010), 35, 1093–1115.
[16] دکتر مرتضی دهقان نژاد، "صنعت و تجارت ابریشم ایران در عصر زندیه"، مجله الکترونیکی تاریخ و توسعه دانشگاه صنعتی اصفهان، 1388.
[17] B.H. Stuart,” Infrared Spectroscopy: Fundamentals and Applications”, England, John Wiley & Sons, (2004),
[18] T. Miyazawa, E.R. Blout,” The Infrared Spectra of Polypeptides in Various Conformations: Amide I and II Bands”, Journal of American Chemical Society (1961), 83, 712–719.
[19] B.B. Mandal, S.C. Kundu,” Non-Bioengineered Silk Gland Fibroin Protein: Characterization and Evaluation of Matrices for Potential Tissue Engineering Applications”, Biotechnology and Bioengineering (2008), 6, 1237–1250.
[20] I.C. Um, H.Y. Kweon, Y. H. Park, S. Hudson,” Structural characteristics and properties of the regenerated silk fibroin prepared from formic acid”, International Journal of Biological Macromolecules(2001), 29, 91–97.
[21] J. Ayutsedea, M. Gandhib, S. Sukigarac, M. Micklusa, H. Chend, F. Ko,” Regeneration of Bombyx mori silk by electrospinning Part 3: characterization of electrospun nonwoven mat”, Polymer (2005), 46, 1625–1634.
[22] Z. H. Zhu, K. Ohgo, T. Asakura, “Preparation and characterization of regenerated Bombyx mori silk fibroin fiber with high strength”, Express Polymer Letters (2008), 12, 885–889.
[23] Y. Iridag, M.t Kazanci,” Preparation and Characterization of Bombyx mori Silk Fibroin and Wool Keratin”, Journal of Applied Polymer Science (2006), 5, 4260–4264.