Fabrication Of A Porous Composite Scaffold For Bone Tissue Engineering Based On Gelatin And Hydroxyapatite, Part I: Cell Culture Results

Asgarzadeh Tabrizi, Karim; Ourang, Fariba

doi:10.22041/ijbme.2004.13516

Document Type : Full Research Paper

Authors

¹ Department of Biomedical Engineering, AmirKabir University of Technology

² Biomaterials Group, Department of Biomedical Engineering, Amir Kabir University of Technology

10.22041/ijbme.2004.13516

Abstract

Gelatin is a protein which is derived from the organic constituent of bone (collagen). Combination of this protein with the inorganic constituent of bone (hydroxyapatite) may provide closer properties to the natural bone. In this study, a biodegradable composite scaffold based on gelatin and hydroxyapatite was prepared as a substitute for bone tissue. To increase the biocompatibility of this, composite, its fabrication was carried out without using any organic solvent. Porosities obtained were spontaneously achieved without any porogen. The pore morphology indicated a high interconnectivity with diameters ranging from 50 to 200 micrometers, which seems appropriate for bone tissue engineering applications. In order to study the biocompatibility of the scaffolds, mouse fibroblastic cells were used. After 24-hour cell culture period in vitro, suitable cell attachment was observed showing high biocompatibility for all the samples. Further examinations demonstrated that the best biocompatibility is obtained for the composite of 50 wt% hydroxyapatite and 50 wt% gelatin.

Keywords

Main Subjects

Tissue Engineering

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Iranian Journal of Biomedical Engineering

Fabrication Of A Porous Composite Scaffold For Bone Tissue Engineering Based On Gelatin And Hydroxyapatite, Part I: Cell Culture Results

References

References

Volume -1, Issue 1 - Serial Number 1
June 2004
Pages 57-64

Fabrication Of A Porous Composite Scaffold For Bone Tissue Engineering Based On Gelatin And Hydroxyapatite, Part I: Cell Culture Results

References

References

Volume -1, Issue 1 - Serial Number 1June 2004Pages 57-64

Volume -1, Issue 1 - Serial Number 1
June 2004
Pages 57-64