نوع مقاله: مقاله کامل پژوهشی

نویسندگان

1 دانشکده مهندسی پزشکی، دانشگاه صنعتی امیرکبیر

2 دانشکده مهندسی پلیمر، دانشگاه صنعتی امیرکبیر - پژوهشگاه پلیمر و پتروشیمی ایران

3 مرکز تحقیقات مهندسی ژنتیک و بیوتکنولوژی

4 پژوهشگاه لیزر، سازمان انرژی اتمی ایران

10.22041/ijbme.2007.13483

چکیده

اخیرا مطالعاتی در زمینه توسعه روش های جدید برای افزایش چسبندگی باکتریایی روی سطوح پلیمری وجود دارد که در کاربرد های بیولوژیکی از جمله بیوسنسور های پایه سلولی استفاده می شوند. در این مطالعه، سطح فیلم پلی اتیلن ترفتالات (PET) در معرض نور لیزر های اکسایمر کریپتون فلوئوراید (KrF) و دی اکسید کربن (CO2) ضربه ای قرار گرفت و رفتار چسبندگی باکتری اشرشیاکلی (E-coli K-12) روی سطوح پرتو دهی شده در شرایط آزمایشگاهی (in virto) بررسی شد. تغییرات خواص سطح PET حاصل از پرتو دهی لیزر، توسط میکروسکوپ الکترونی رویشی (SEM) و اندازه گیری زاویه تماس مشخص شد. نتایج نشان داد که آمایش لیزری باعث تغییر مورفولوژی و همچنین آبدوستی سطح PET می شود. تعداد باکتری های E-coil K-12 که به سطح PET چسبیده بودند به طور کمی توسط رنگ آمیزی فلورسنت، مشاهدات میکروسکوپی و شمارش از طریق نرم افزار Image Proplus بررسی شد. نتایج نشان داد که تعداد باکتری های E-coil K-12چسبیده به سطوح پرتو دهی شده با لیزر های کریپتون فلورئورید و دی اکسید کربن در مقایسه با سطوح اصلاح نشده افزایش یافت.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

In Vitro Evaluation Of Escherichia Coli K–12 Bacteria Adhesion Onto Co2 And Krf Laser-Treated Polyethylene Terephthalate (Pet)

نویسندگان [English]

  • Ruhollah Mahdinavaz Maghdam 1
  • Hamid Mirzadeh 2
  • Morteza Daliri 3
  • Alireza Zare 4

1 Faculty of Biomedical Engineering, Amirkabir University of Technology

2 Department of Polymer Engineering, Amirkabir University of Technology - Iran Polymer and Petrochemical Institute

3 Biotechnology & Genetic Engineering Research Center

4 Atomic Energy Organization of Iran

چکیده [English]

Recently there are studies in developing new methods to increase bacterial adhesion onto polymeric surfaces that are used in biological application such as cell-based biosensors. In this study the surface of polyethylene terephthalate (PET) films were irradiated using CO2 and KrF excimer pulsed lasers and adhesion behavior of Escherichia coli k-12 (E. coli K-12) bacteria onto the irradiated surfaces was studied in vitro. The changes in the surface properties due to laser irradiation were characterized by scanning electron microscopy (SEM) and contact angle measurement. The results showed that laser treatment changes surface morphology and surface hydrophilicity. The number of bacteria that were adhered onto the surfaces was quantitatively investigated by fluorescent staining, microscopic observations and counting through Image Proplus software. The results showed that the number of adhered E. coli K-12 bacteria onto the irradiated surfaces by both CO2 and KrF lasers in comparison with unmodified surfaces was increased.

کلیدواژه‌ها [English]

  • Krf Laser
  • Co2 Laser
  • Laser Treatment
  • Polyethylene terephthalate
  • Bacterial Adhesion

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