1دانشجوی کارشناسی ارشد، دانشکده‌ی مهندسی مکانیک، دانشگاه علم و صنعت ایران، تهران
2استادیار، دانشکده‌ی مهندسی مکانیک، دانشگاه علم و صنعت ایران، تهران
3دانشجوی دکتری، دانشکده‌ی مهندسی مکانیک، دانشگاه علم و صنعت ایران، تهران
چکیده
تولید و استفاده از وسایل تشخیصی-تحلیلی میکروسیالی، به ویژه وسایل تحلیلی میکروسیالی کاغذی (میکروپدها)، به دلیل مزایای فراوان آن‌ها، از جمله هزینه‌ی عملیاتی پایین، مصرف کم نمونه و عدم نیاز به مهارتی خاص برای استفاده و بازیافت یا دفع آسان، مورد استقبال زیادی قرار گرفته است. میکروپدها بدون نیاز به نیروی محرک خارجی، توانسته‌اند در تشخیص بسیاری از بیماری‌ها موفق ظاهر شوند. هدف این مقاله، توسعه‌ی یک میکرومیکسر برای تشخیص بر مبنای رنگ‌سنجی نیتریت موجود در بزاق به کمک واکنش گریس و افزایش بازه‌ی حد تشخیص دستگاه به کمک بهبود اختلاط می‌باشد. میکرومیکسرها پس از یک طراحی ساده، با برش لیزر ساخته شدند. در این کار، پنج هندسه‌ی متفاوت، شامل ساده، مارپیچ، زیگ‌زاگ، شکسته‌ی قائم و شکسته‌ی مایل، برای بخش اختلاط میکرومیکسر، به دو صورت تجربی و شبیه‌سازی، آزمایش و مقایسه شده‌اند. شبیه‌سازی با مدل جریان دوفازی همگن در محیط متخلخل، در نرم‌افزار انسیس سی‌اف‌اکس صورت گرفته است. نتایج نشان دادند که میکرومیکسر شکسته‌ی مایل نسبت به میکرومیکسر ساده، با بهبود 24/44 درصدی، بهترین عمل‌کرد را در آزمایش داشته، که حاصل آن محدوده‌ی خطی تشخیص صفر تا 1000 molL-1μ و حد تشخیص 7/5 molL-1μ شده است. بدین ترتیب، با توجه به تاثیر فراوان میکرومیکسرها در میکروسیالات، به کمک اصلاحات هندسی ساده، کیفیت اختلاط و در نتیجه دقت وسیله‌ی اندازه‌گیری افزایش داده شد.
Nitrite Colorimetric Detection in Saliva using Optimal Mixing
نویسندگان [English]
Elham Mollaei1؛ Sasan Asiaei2؛ Mahdi Moghimi2؛ Ali Zadekafi3
1M.Sc Student, Mechanical Engineering Department, Iran University of Science and Technology, Tehran, Iran
2Assistant Professor, Mechanical Engineering Department, Iran University of Science and Technology, Tehran, Iran
3PhD Student, Mechanical Engineering Department, Iran University of Science and Technology, Tehran, Iran
چکیده [English]
Microfluidic analytical/diagnostic tools, especially microfluidic paper-based analytical devices ( PADs) have attracted considerable attention due to their numerous advantages including their low operational costs, small analyte consumption, and limited required skills for use, and easy disposal/recycling. μPADs have been successful in detection of various diseases with no external deriving units. The aim of this study is to develop a micromixer for colorimetric detection of nitrite in saliva using Griess reaction and widening the limit of detection (LOD) by mixing improvement. Micromixers were fabricated using laser cut after a simple design. Five different geometries were examined and compared including straight, curved, zigzag, square wave and hexagonal, by numerical simulation and experimental tests for mixing part in micromixer. Simulations were performed in ANSYS CFX with homogeneous two-phase flow model in a porous media. As the result inclined hexagonal micromixer showed the best performance (in comparison with the straight one) exhibiting 44.24% of improvement which leads to a detection range and LOD of and , respectively. Considering the significant impact of micromixers in microfluidics, the quality of mixing and therefore the accuracy of the devices was improved by simple geometrical modifications.
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