Synthsis Of Cdse/Zns Core Shell Quantum Dots And In Vitro Evaluation Of Their Emission Effects On Raji Cells Death

Parviz, Maryam; Rabiee, Mohammad; Shokrgozar, Mohammad Ali; Moztarzadeh, Fathollah; Yousefi Gharebaghi, Farzad; Shayan, Mahdis; Tahriri, Mohammad Reza

doi:10.22041/ijbme.2009.13387

Document Type : Full Research Paper

Authors

¹ M.Sc Student, Biomaterial Group, School of Biomedical Engineering, Amirkabir University of Technology

² Associate Professor, Biomaterial Group, School of Biomedical Engineering, Amirkabir University of Technology

³ Associate Professor, National Cell Bank of Iran, Pasteur Institute of Iran

⁴ Professor, Biomaterial Group, School of Biomedical Engineering, Amirkabir University of Technology

⁵ M.Sc Graduated, Biomaterial Group, School of Biomedical Engineering, Amirkabir University of Technology

⁶ PhD Candidate, Biomaterial Group, School of Biomedical Engineering, Amirkabir University of Technology

10.22041/ijbme.2009.13387

Abstract

A great deal of attention has been drawn to the colloidal chemistry based semiconductor nanocrysallites, also known as quantum dots (QDs). Because of the strong quantum confinement, quantum dots have unique size-dependent optical properties, which are much more superior to the conventional organic fluorescence materials. In addition, strong chemical resistant makes inorganic semiconductor QDs an ideal candidate for next-generation of bio-labels and other biomedical applications. This study presents a synthesize method of high quality biocompatible CdSe QDs in aqueous solution by using gelatin as inhibitor. A thin ZnS layer was grown on CdSe QDs, forming a CdSe/ZnS core/shell structure, to improve the photoluminescence. The optical properties of the QDs were characterized by photoluminescence spectra. The stability of CdSe/ZnS QDs in aqueous solution has been improved with capping in mercaptosuccinic acid (MSA) groups. PAGE test results show that carboxylic groups of MSA have interacted with NH2 agent of CD20 antibodies. We evaluate the in vitro efficiency of the luminescence of the bioconjugated QDs to kill Raji cells. Raji cells have anti-CD20 which can especially connect to CD20-QDs. In conclusion, we found that CD20-CdSe/ZnS bioconjugated QDs with different concentration 50-250 µg/mL which have been exited at 400nm in 2h intervals can result in Raji cells death. This study shows the potential of CdSe/ZnS for cancer treatment.

Keywords

References

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Iranian Journal of Biomedical Engineering

Synthsis Of Cdse/Zns Core Shell Quantum Dots And In Vitro Evaluation Of Their Emission Effects On Raji Cells Death

References

References

Volume 3, Issue 2 - Serial Number 2
June 2009
Pages 89-97

Synthsis Of Cdse/Zns Core Shell Quantum Dots And In Vitro Evaluation Of Their Emission Effects On Raji Cells Death

References

References

Volume 3, Issue 2 - Serial Number 2June 2009Pages 89-97

Volume 3, Issue 2 - Serial Number 2
June 2009
Pages 89-97