Synthesis Of Magnesium Calcium Phosphate Nanoparticles Using Biomimetic Method In Amino Acids Environment

Kazemzadeh, Rouzbeh; Behnamghader, Ali Asghar; Hesaraki, Saeed; Hazrati, Fateme

doi:10.22041/ijbme.2009.13393

Document Type : Full Research Paper

Authors

¹ M.Sc Student, Biomaterial Group, Materials and Energy Research Center

² Assistant Professor, Biomaterial Group, Materials and Energy Research Center

³ M.Sc Student, Biomedical Engineering School, Science and Research Branch of Islamic Azad University

10.22041/ijbme.2009.13393

Abstract

Magnesium-contained Hydroxyapatite Nano powder was synthesized by wet chemical method using calcium nitrate tetra hydrate, magnesium nitrate hexa hydrate and di ammonium hydrogen phosphate in the presence of Glutamic acid. According to thermal analysis (STA) findings the samples were calcinated at specific temperatures and characterized by XRD, FTIR and TEM analysis. XRD results showed the that b-TCP ((Ca1-xMgx)3(PO4)2) was the dominant phase at 920°C. No characteristic peaks of hydroxyapatite were observed at that temperature. In contrast, the sample which was synthesized in the absence of Glutamic acid, contained both hydroxyapatite and b-TCP phase. The Findings showed a rapid decline in degree of crystallinity at 90°C with presence of Glutamic acid in reaction media. Transmission electron microscopy (TEM) observations on heat treated samples at 480°C revealed that using Glutamic acid has noticeable effect on crystallite size instead of its growth orientation. Dimensions of biomimetic nanoparticles as observed by TEM were 150x60nm and in the witness sample was 500x150nm. According to Scherrer formula for crystallite size, the size of the witness sample was calculated about 40nm. However, because of low degree of crystallinity it was impossible to calculate the size of Glutamic contained samples.

Keywords

Main Subjects

Nano-Biomaterials

References

[1] Lee D., Sfeir C., Kumta P.N., Novel in-situ synthesis and characterization of nanostructured magnesium substituted β tricalcium phosphate (β-TCMP); Materials Science and Engineering, 2009; 29: 69–77.

[2] Bianco A., Cacciotti I., Lombardi M., Thermal stability and sintering behavior of hydroxyapatite nanopowders; Thermal Analysis and Calorimetry, 2007; 88: 237– 243.

[3] Landi E., Logroscino G., Proietti L., Tampieri A., Biomimetic Mg-substituted hydroxyapatite: from synthesis to in vivo behavior; Mater Sci: Mater Med, 2008; 19:239–247.

[4] Kannan S., Lemos I.A.F., Rocha J.H.G., Ferreira J.M.F., Synthesis and characterization of magnesium substituted biphasic mixtures of controlled hydroxyapatite/β-tricalcium phosphate ratios; Solid State Chemistry, 2005; 178: 3190–3196.

[5] Gray C., The Cyborg Handbook; Routledge New York; 2003, 12-16.

[6] Benyus J., Biomimicry: Innovation Inspired by Nature; Harper Perennial, New York, 2006, 212- 223.

[7] Zhang W., Huang Z.L., Nucleation Sites of Calcium Phosphate Crystals during Collagen Mineralization; Biomaterials Laboratory. J. Am. Ceram Soc, 2000; 86: 1052–54.

[8] Matsumoto T., Okazaki M., Inoue M., Hamada Y., Crystallinity and solubility characteristics of hydroxyapatite adsorbed amino acid; Biomaterials, 2002; 23: 2241–2247.

[9] McQuire R., Ching J., Vignaud E., Lebugle A., Synthesis and characterization of amino acidfunctionalized hydroxyapatite nanorods; J. Materials Chemistry, 2004; 14: 277–2281.

[10] Cacciotti I., Bianco A., Lombardi M., Mg-substituted hydroxyapatite nanopowders: Synthesis, thermal stability and sintering behavior; J of the European Ceramic Society, 2008; 29: 2969–2978.

[11] Jenkins R., Snyder R.L., Introduction to X-Ray Powder Diffractometry; John Wiley & Sons, New York, 1996; 68-70

[12] Suchaneka W.L.; Byrappaa K.; Shuk P.; Preparation of magnesium-substituted hydroxyapatite powders by the mechanochemical–hydrothermal method, Biomaterials, 2004; 25: 4647–4657.

[13] Marchi J., Dantas A.C.S., Greil P., Influence of Mgsubstitution on the physicochemical properties of calcium phosphate powders, Materials Research Bulletin, 2007; 42: 1040– 1050.

[14] Boanini E., Fini M., Gazzano M., Bigi A., Hydroxyapatite Nanocrystals Modified with Acidic Amino Acids; Eur. J. Inorg. Chem, 2006: 4821–4826.

[15] Chirantha R., Synthesis and characterization of strontium Fluorapatite; Departement of Chemistry College of Sciences, Graduate College University of Nevada, Las Vegas, Augest 2005.

[16] Matsumoto T., Okazaki M., Inoue M., Hamada Y., Crystallinity and solubility characteristics of hydroxyapatite adsorbed amino acid; Biomaterials, 2002; 23: 2241–2247.

[17] Aßmanna S., Viertelhaus M., Heiß A., Hoetzer K.A., The influence of amino acids on the biomineralization of hydroxyapatite in Gelatin; Journal of Inorganic Biochemistry, 2002; 91: 481–486.

Iranian Journal of Biomedical Engineering

Synthesis Of Magnesium Calcium Phosphate Nanoparticles Using Biomimetic Method In Amino Acids Environment

References

References

Volume 3, Issue 2 - Serial Number 2
June 2009
Pages 127-133

Synthesis Of Magnesium Calcium Phosphate Nanoparticles Using Biomimetic Method In Amino Acids Environment

References

References

Volume 3, Issue 2 - Serial Number 2June 2009Pages 127-133

Volume 3, Issue 2 - Serial Number 2
June 2009
Pages 127-133